FPCount protocol - in-lysate (purification free) protocol

Abstract

FPCount is a complete protocol for fluorescent protein calibration, consisting of 1. FP expression and production of cell lysates. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. Results can be analysed with the corresponding R package, FPCountR. This in-lysate version of the protocol uses the ECmax protein quantification protocol of FPs in lysates and does not require His-tag purification of the FPs.