Protein metrology and biological circuit control

I am currently a Research Associate in Synthetic Biology in the group of Professor Guy-Bart Stan at the Centre for Synthetic Biology (CSynB), Department of Bioengineering, Imperial College London.

FPCountR: My recent focus has been the development and publication of a method for the calibration of plate readers that allows the typical microbial synthetic biology experiment to be converted from ‘relative fluorescence units’ to more meaningful, absolute numbers - such as molecules of GFP per cell. This work comprises a detailed laboratory protocol, validation of which was detailed in our recent paper, as well as an accompanying R package which automates the analysis of the calibration data as well as subsequent experimental data.

The ultimate aim is to use these methods to enable high-throughput screening of circuits optimised for a variety of protein production scenarios in microbial biotechnology, particularly where cellular resource use is a challenge and protein level regulation requires careful control.

Eszter Csibra
Eszter Csibra
Lecturer in Bacteriology