Wet Lab Resources for Molecular Biologists

2023-08-01

Last updated August 2023.

For the version that I keep updated, see GitHub.

Here are a list of online resources that should help you find information about DNA part sequences, molecular biology questions or basic protocols for handling DNA/RNA/proteins and E. coli, and to help you to begin to troubleshoot your own experiments. Most resources are freely available. Note that this list isn’t meant to be exhaustive, it is mainly to get you started.

General Reference and Tools

MolBio Reference and Tools

Codes and abbreviations

Codon usage tables

Tools

General Lab Techniques

Buffers

Protocol databases

Protocol FAQs

Molecular Biology Techniques

CLONING - DNA Construct Design

Software:

Plasmids:

Plasmid databases:

Plasmid parts: The vector

Plasmid parts: The construct

Cloning methods:

Primer design:

DNA sequence optimisation tools:

E. coli strains:

CLONING - In Practice

General cloning protocols:

Essential basic protocols:

Reagent and kit-specific information:

  • Reagent-specific information can be found on the manufacturer’s website.
  • Kits: Minipreps, PCR purification, Gel extraction - All include manuals within the boxes, which can also be found online.

More information:

MICROBIOLOGY

E. coli strains/genotypes:

E. coli genome/genes:

E. coli general:

PROTEIN EXPRESSION (E. coli)

Optimisation (maximisation):

Protein electrophoresis:

Troubleshooting:

PROTEIN PURIFICATION

Cell lysis and clarification:

Purification techniques:

Concentration analysis:

Storage:

PROTEIN ANALYSIS

Protein structure databases

Amino acid properties

Protein Properties

Protein families by structure

BIOCHEMISTRY/CHEMISTRY

FLUORESCENT PROTEINS

EXPERIMENTAL DESIGN

ANALYSIS

FIGURES

Photos and Diagrams:

Data visualisation:


Image credit: Unsplash

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Eszter Csibra
Eszter Csibra
Lecturer in Bacteriology