directed-evolution

Directed Evolution

My work at UCL on directed evolution methods, using bacterial surface display for xenobiology.

Bacterial cell display as a robust and versatile platform for the engineering of low affinity ligands and enzymes

Directed evolution has been remarkably successful at expanding the chemical and functional boundaries of biology. That progress is heavily dependent on the robustness and flexibility of the available selection platforms, given the significant cost to …

E. coli surface display of streptavidin for directed evolution of an allylic deallylase

Artificial metalloenzymes (ArMs hereafter) combine attractive features of both homogeneous catalysts and enzymes and offer the potential to implement new-to-nature reactions in living organisms. Herein we present an E. coli surface display platform …

Selection platforms for directed evolution in synthetic biology

Life on Earth is incredibly diverse. Yet, underneath that diversity, there are a number of constants and highly conserved processes- all life is based on DNA and RNA; the genetic code is universal; biology is limited to a small subset of potential …