fluorescent-proteins

Absolute protein quantification using fluorescence measurements with FPCountR

This paper presents a generalisable method for the calibration of fluorescence readings on microplate readers, in order to convert arbitrary fluorescence units into absolute units. FPCountR relies on the generation of bespoke fluorescent protein (FP) …

FPCountR- improved analytical methods enable absolute protein quantification

Contributed abstract to the 14th International Workshop on Bio-Design Automation, Paris, France, October 24th-26th 2022.

Beyond fluorescein- Use of fluorescent protein calibrants for direct and absolute quantification of protein production in synthetic biology.

FPCount protocol - in-lysate (purification free) protocol

FPCount is a complete protocol for fluorescent protein calibration, consisting of 1. FP expression and production of cell lysates. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. …

FPCountR- Fluorescent protein calibration for plate readers

The R package FPCountR enables the calibration of microplate readers using fluorescent protein calibrants. This enables users to monitor cellular fluorescent protein expression in absolute units. More information is available on the GitHub page …